Journal: International journal of molecular sciences

Article Title: Effect of Airborne Particulate Matter on the Immunologic Characteristics of Chronic Rhinosinusitis with Nasal Polyps.

doi: 10.3390/ijms23031018

Figure Lengend Snippet: Figure 5. The pivotal effect of PM10-induced IL-33 for Th2 differentiation effects of conditioned media on PM10-induced human nasal polyp derived fibroblast (NPDFs-CM) on the inflammatory cytokine production in CD4+ T cells. (A) Th2 differentiation was determined by the expression of GATA3 and ST2 (IL-33 receptor). (B,C) The protein levels of IFN-γ, IL-17A, IL-4, IL-13, and IL-10 in CD4+ T cell populations were determined by flow cytometry according to the addition of ST2. The graphic data represents the means ± SEM (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.0001 as compared to the PM10-treated group.

Article Snippet: TMCs (1 × 106 cells) were cultured with 1 μg/mL of phytohemagglutinin (PHA) in NPDF-CM for 72 h. For Th2 differentiation, CD4+ T cells from TMCs were purified using a CD4+ T Cell Isolation Kit MicroBeads (Miltenyi Biotech, Bisley, Surrey, UK), and purified CD4+ T cells were differentiated using a CellXVivo Human Th2 Cell Differentiation Kit (RnDSystems, Minneapolis, MN, USA) following the manufacturer’s instructions.

Techniques: Derivative Assay, Expressing, Cytometry

Journal: Hepatology Communications

Article Title: Hyperoxidized Albumin Modulates Platelets and Promotes Inflammation Through CD36 Receptor in Severe Alcoholic Hepatitis

doi: 10.1002/hep4.1440

Figure Lengend Snippet: Validation of platelet functionality in SAH. (A) Platelet activation/aggregation in SAH histogram shows the percent expression of PAC‐1, P‐selectin (CD62p), and intracellular Ca +2 (secretion) in SAH and HC. (B) Increased expression (relative change) of various mRNA in SAH platelets linked to platelet and complement activation. Protein expression of Gp2b/3a/β‐actin ratio in platelets of patients with SAH. Results expressed as mean ± SEM (* P < 0.05, ** P < 0.01 for SAH versus HC). (C) Patients with SAH showed higher plasma levels of PF‐4 (65.85 ± 0.9457 versus 81.13 ± 6.595 ng/mL, P < 0.02), P‐selectin (6.360 ± 0.1639 versus 10.118 ± 2.1492 ng/mL, P < 0.0001), and soluble cluster of differentiation 40 ligand (1,726 ± 134.4 versus 2,528 ± 1.525.4 pg/mL, P < 0.05). Circulating levels of PF‐4, P‐selectin, and sCD40L correlated with the MELD score in SAH (r > 0.5, P < 0.05). (D) Representative western blot of SNARE complex proteins in platelets isolated from SAH versus HC (n = 5). Results are expressed as mean ± SEM (* P < 0.05, ** P < 0.01, *** P < 0.001 as indicated).

Article Snippet: Plasma‐citrated blood samples were centrifuged for 15 minutes at 3,000 g , and levels of PF‐4 (E‐EL‐H2216; Elabscience, Houston, TX), P‐selectin (E‐EL‐H0917; Elabscience), and sCD40L (E‐EL‐H0035; Elabscience) were measured.

Techniques: Biomarker Discovery, Activation Assay, Expressing, Clinical Proteomics, Western Blot, Isolation

Journal: Cell Communication and Signaling : CCS

Article Title: GDF9 His209GlnfsTer6/S428T and GDF9 Q321X/S428T bi-allelic variants caused female subfertility with defective follicle enlargement

doi: 10.1186/s12964-024-01616-8

Figure Lengend Snippet: Effects of GDF9 variants on gene expression and GDF9-BMP15 interactions. A Western blots of mature GDF9 proteins expressed in follicular fluid collected from the first and second retrieval cycle of individual P1 and age-matched controls ( n =3). B ELISA of GDF9 in follicular fluid collected from the first retrieval cycle of individual P1 and age-matched controls ( n =6). C Western blots of GDF9 expression in HEK293T cells transfected with human GDF9 WT , GDF9 Q321X , GDF9 S428T , and GDF9 His209GlnfsTer6 respectively and their relative mature-GDF9 expression in culture medium (CM) (3 independent experiments). The amount of concentrated culture medium used for the detection was 35μl per sample per assay. lnfsTer6, His209GlnfsTer6. * P < 0.05. D Western blots of GDF9 precursors and mature GDF9 over time during in vitro cleavage assay (3 independent experiments). Total GDF9=GDF9 precursor+mature GDF9. E Western blots of BMP15 expression in HEK293T cells co-transfected with human BMP15 and human GDF9 WT , GDF9 Q321X , GDF9 S428T , and GDF9 His209GlnfsTer6 respectively and their relative BMP15 precursor expression in CM (3 independent experiments). lnfsTer6, His209GlnfsTer6. * P < 0.05. F Co-IP assay of the binding of GDF9 proteins to BMP15 protein. lnfsTer6, His209GlnfsTer6. * P < 0.05. G Western blots of p-smad2 and smad2 expression in human primary luteinized granulosa cells treated with purified recombinant pro-GDF9 WT -BMP15 and pro-GDF9 S428T -BMP15 protein respectively (3 independent experiments)

Article Snippet: GDF9 was measured in follicular fluid collected at the first oocyte retrieval from individual P1 and in follicular fluid from other six control women using an ELISA kit (CSB-E12925h, CUSABIO, China).

Techniques: Gene Expression, Western Blot, Enzyme-linked Immunosorbent Assay, Expressing, Transfection, In Vitro, Cleavage Assay, Co-Immunoprecipitation Assay, Binding Assay, Purification, Recombinant